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Indexed by:期刊论文

Date of Publication:2008-08-13

Journal:JOURNAL OF ETHNOPHARMACOLOGY

Included Journals:SCIE、PubMed、Scopus

Volume:118

Issue:3

Page Number:495-502

ISSN No.:0378-8741

Key Words:Acanthopanax senticosus; Araliaceae; anti-inflammatory effect; tumor necrosis factor-alpha; interleukin-10; nuclear factor-kappa B

Abstract:Aim of the study: In this study, we evaluated protective effect of Acanthopanox senticosus extract (ASE) and a possible signaling pathway involved during endotoxic shock induced by intraperitoneal injection lipopolysaccharide (LPS) and D-galactosamine (D-GaIN) in BALB/c mice.
   Materials and methods: Mice were intraperitoneal administrated with ASE (100, 200 or 400 mg/kg) prior to injection of 50 mu g/kg LPS and 1 g/kg D-GaIN. The levels of tumor necrosis-alpha (TNF-alpha) and interleukin-10 (IL-10) in serum and liver. Nitric oxide (NO) production in serum and inducible nitric oxide synthase (iNOS) protein level were investigated. Nuclear factor-kappa B (NF-kappa B) activation in liver was determined. Furthermore, we evaluated the effect of ASE pretreatment on infiltration of inflammatory cells into the heart, liver and lung of mice.
   Results: Treatment of mice with ASE prior to LPS/D-GaIN injection significantly improved the survival rate. ASE pretreatment inhibited the elevation of TNF-alpha in serum and liver. ASE also decreased iNOS level in liver and the overproduction of nitric oxide (NO) in serum. In addition, IL-10 levels in serum and liver were markedly enhanced. ASE pretreatment inhibited NF-kappa B activation in liver of mice. Moreover, infiltration of inflammatory cells into the heart, liver and lung of mice was also attenuated by ASE pretreatment.
   Conclusions: These results suggested that ASE protected mice against LPS/D-GaIN-induced endotoxic shock involving inhibition of NF-kappa B activation, which caused down-regulation of TNF-alpha and involved up-regulation of IL-10. Acanthopanax senticosus may thus prove beneficial in the prevention of endotoxic shock. (c) 2008 Elsevier Ireland Ltd. All rights reserved.