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论文类型：期刊论文

发表时间：2017-12-01

发表刊物：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT

收录刊物：SCIE

卷号：53

期号：6

页面范围：561-570

ISSN号：1054-5476

关键字：Antioxidant capacity; Dianthus chinensis L.; Hyperhydricity; Silver nitrate; Water loss

摘要：Hyperhydricity symptoms are common and significant during the in vitro culture of Dianthus chinensis L. and greatly affect the micropropagation and regeneration of cultured plantlets. However, effective measures for preventing such abnormalities have not been developed for this species. Silver nitrate (AgNO3) has been shown to revert hyperhydric plantlets to a normal state. Nevertheless, the effect of AgNO3 on the prevention of hyperhydricity and the underlying mechanisms remain unclear. In the present study, 98.7% of the Dianthus chinensis L. plantlets cultured in a hyperhydricity induction medium (HIM) developed symptoms of hyperhydricity; however, hyperhydricity symptoms were inhibited to different degrees when D. chinensis L. plantlets were cultured in HIM supplemented with various concentrations of AgNO3. In particular, approximately 97% of the D. chinensis L. plantlets grew normally and did not show any symptoms of hyperhydricity when cultured in HIM supplemented with 30 mu mol L-1 AgNO3. Compared with the plantlets cultured in HIM alone, the plantlets cultured in HIM containing AgNO3 displayed dramatic decreases in water content, ethylene content, and reactive oxygen species (ROS) production (particularly regarding H2O2 accumulation in guard cells) and showed increased antioxidant enzyme activity, stoma aperture, and water loss. These changes not only prevented excess water from accumulating in the tissues of plantlets but also improved the antioxidant capacity of plantlets, ultimately resulting in the prevention of hyperhydricity.