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论文类型：期刊论文

发表时间：2012-12-01

发表刊物：PLANT CELL TISSUE AND ORGAN CULTURE

收录刊物：EI、SCIE、Scopus

卷号：111

期号：3

页面范围：393-397

ISSN号：0167-6857

关键字：Chrysanthemum (Yuukou); Somatic embryogenesis; Leaf and stem explant; Plant regeneration; Structure analysis

摘要：Somatic embryogenesis was induced from leaf and stem of chrysanthemum (cv. Yuukou) using various combinations of plant growth regulators. About 93 and 63 % of somatic embryogenesis were respectively induced from leaf and stem cultured in Murashige and Skoog (MS) medium supplemented with 6-benzyladenine (BA) and a-naphthalene acetic acid (NAA). Somatic embryogenesis was more readily induced from leaf, yielding 100 % regeneration rate in MS medium supplement with 0.6 mg/L BA and 1.8 mg/L NAA. Plants were efficiently regenerated from leaf in medium lacking growth regulators, yielding a regeneration rate of 98 %. Micro-morphology analysis revealed the presence of individual somatic embryo and there is no vascular bundle connection between the new embryo structure and the parental tissue. In this study, an efficient system for plant regeneration via somatic embryogenesis from leaves and stems of chrysanthemum was achieved. The result may facilitate mass production of high-quality chrysanthemum seedings for the commercial market.