Release Time:2025-11-20  Hits:

Date of Publication: 2022-10-09

Journal: 药学学报

Institution: 生物工程学院

Volume: 49

Issue: 7

Page Number: 1062-1068

ISSN: 0513-4870

Abstract: The microstructure of cationic cyclopeptide (TD-34) treated Caco-2 cell
   membrane was observed, and we discussed the relationship between
   membrane structure and insulin transmembrane permeability. Atomic force
   microscope (AFM) was used to observe living cell membrane in air
   condition and tapping mode. Results showed that the surface of Caco-2
   cell membrane treated with TD-34 lost its smoothness and nearly doubled
   its roughness. Apparent permeability coefficients (P_(app)) of insulin
   in Caco-2 cell monolayers increased 2.5 times. In conclusion, AFM can be
   used to observe microstructure of cationic cyclopeptide treated cell
   membrane and cationic cyclopeptide enhanced insulin delivery across
   Caco-2 cell membrane by increasing membrane fluidity.

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