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盾叶薯蓣糖化液发酵生产2,3-丁二醇

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Indexed by:期刊论文

Date of Publication:2009-02-15

Journal:过程工程学报

Included Journals:Scopus、PKU、ISTIC、CSCD

Volume:9

Issue:1

Page Number:95-100

ISSN No.:1009-606X

Key Words:克雷伯氏杆菌;盾叶薯蓣;2,3-丁二醇;代谢流量分布

Abstract:利用克雷伯氏杆菌以盾叶薯蓣糖化液为底物发酵生产2,3-丁二醇-2,3-BD),考察了2,3-BD浓度、生产强度、有机酸生成及代谢流量分布情况. 结果表明,盾叶薯蓣中的有机酸成分能促进三羧酸循环途径和乙酸途径的代谢流,减弱琥珀酸途径的代谢流,从而提高2,3-BD的浓度. 以盾叶薯蓣糖化液为底物,采用批式流加方式,补加固体葡萄糖,发酵56 h,发酵液中2,3-BD最终浓度达到80.20 g/L,乙偶姻与2,3-BD浓度之和最终达到86.19 g/L,生产强度达到1.54 g/-L(h),比单独以葡萄糖为底物时分别提高了8.50%, 7.38%和7.69%.

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