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论文类型：期刊论文

发表时间：2019-07-14

发表刊物：ANALYTICAL METHODS

收录刊物：SCIE、EI

卷号：11

期号：26

页面范围：3350-3357

ISSN号：1759-9660

摘要：Highly specific and sensitive detection of hepatitis A virus (HAV) in water is of great significance for drinking water safety and epidemic control. In this work, a hybrid nanobiosensor for HAV detection was established based on fluorescence resonance energy transfer (FRET) between quantum dots (QDs) as a donor and Cy5 as an acceptor. Transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy and dynamic light scattering were used to characterize the chemical and physical structures of the hybrid biosensor. Under optimized conditions, the detection limit of target DNA (tDNA) was 1.3 x 10(-11) mol L-1 (S/N = 3), with a linear range of 2 x 10(-10) mol L-1 to 5 x 10(-8) mol L-1. In addition, it showed excellent selectivity in the presence of various interfering species. The results show the applicability of the biosensor for monitoring HAV in environmental and food samples.