个人信息Personal Information
副教授
硕士生导师
性别:女
毕业院校:大连理工大学
学位:硕士
所在单位:生物工程学院
电子邮箱:ljchen@dlut.edu.cn
Engineering an industrial Saccharomyces cerevisiae strain with the inulinase gene for more efficient ethanol production from Jerusalem artichoke tubers
点击次数:
论文类型:期刊论文
发表时间:2013-09-01
发表刊物:ENGINEERING IN LIFE SCIENCES
收录刊物:SCIE、EI、Scopus
卷号:13
期号:5
页面范围:472-478
ISSN号:1618-0240
关键字:Consolidated bioprocessing (CBP); Ethanol fermentation; Inulinase; Jerusalem artichoke tubers; Saccharomyces cerevisiae
摘要:Engineering industrial microbial strains with inulinase production for developing a consolidated bioprocessing (CBP) strategy is a desirable solution to biofuel production from Jerusalem artichoke tubers. In this study, the integrative vector pFA6a-rDNA-PGK-INU was generated by fusing the 3.3-kb ribosomal DNA fragment with the phosphoglycerate kinase promoter to regulate the expression of the inulinase gene isolated from Kluyveromyces marxianus, which was then integrated into the chromosome of an industrial Saccharomyces cerevisiae strain for ethanol production from Jerusalem artichoke tubers. Compared to the host strain, no significant difference was observed in the growth of the recombinant yeast, but its inulinase production was enhanced: 22.9 versus 10.6 U/mL for aerobic seed cultures and 14.5 versus 10.0 U/mL for ethanol fermentation, which consequently facilitated the CBP process for ethanol production: 72.5 g/L ethanol produced in a fermentation time of 48 h, while only 67.0 g/L ethanol was produced by the host strain in a fermentation time of 60 h. Thus, the ethanol productivity was increased from 1.12 to 1.51 g/L/h, presenting an increase of 34.8%.