location: Current position: Home >> Scientific Research >> Paper Publications

Ex Vitro Expansion of Human Placenta-Derived Mesenchymal Stem Cells in Stirred Bioreactor

Hits:

Indexed by:Journal Papers

Date of Publication:2009-10-01

Journal:APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Included Journals:SCIE

Volume:159

Issue:1

Page Number:110-118

ISSN No.:0273-2289

Key Words:Mesenchymal stem cells; Stirred bioreactor; Cell expansion; Tissue engineering; Regeneration medicine

Abstract:The high demand of human placenta-derived mesenchymal stem cells (hPDMSCs) for therapeutic applications requires reproducible production of large numbers of well-characterized cells under well-controlled conditions. However, no method for fast hPDMSCs proliferation has yet been reported. In the present study, the feasibility of using a stirred bioreactor system to expand hPDMSCs was examined. hPDMSCs were cultured either in stirred bioreactors or in tissue culture flasks (T-flasks) for 5 days. Total cell density and several parameters of physical microenvironments were monitored in the two culture systems every 24 h. The maintenance of the antigenic phenotype of hPDMSCs before and after culturing in the stirred bioreactor system was cytometrically assessed. Data suggested that the physical microenvironment in the stirred bioreactors was much more favorable than that of the T-flasks. At the end of 144 h culturing, the total cell number was increased 1.73 times from the T-flasks to the stirred bioreactors. In addition, hPDMSCs could maintain their antigenic phenotype when cultured in stirred bioreactors. These results provide the initial assessment for large-scale hPDMSCs production using suspension culture bioreactors.

Pre One:应用搅拌式生物反应器大量扩增人胎盘间充质干细胞的实验研究

Next One:Effects of encapsulated rabbit mesenchymal stem cells on ex vivo expansion of human umbilical cord blood hematopoietic stem/progenitor cells