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大孔吸附树脂分离纯化地黄中梓醇工艺的研究

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Indexed by:期刊论文

Date of Publication:2008-01-15

Journal:中国生物工程杂志

Included Journals:PKU、ISTIC、CSCD

Volume:28

Issue:1

Page Number:65-69

ISSN No.:1671-8135

Key Words:地黄;梓醇;大孔吸附树脂;柱层析;高效液相色谱

Abstract:利用大孔吸附树脂分离提取地黄中梓醇.以地黄粗提液中梓醇含量为指标,高效液相色谱(HPLC)为含量测定方法,考察9种不同极性大孔吸附树脂对梓醇的吸附和解吸附性能,筛选出最佳树脂D101进行分离实验.结果表明,D101大孔吸附树脂的静态吸附容量为69.2mg/g干树脂,其吸附等温线符合Langmuir和Freundlich吸附等温式.采用5%乙醇作为洗脱剂,洗脱液减压浓缩后进行硅胶柱层析分离,氯仿∶甲醇(8∶2)梯度洗脱得到梓醇单体,纯度达90%以上,梓醇得率为6%.

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