Release Time:2019-03-09  Hits:

Indexed by: Journal Article

Date of Publication: 2011-08-26

Journal: NEUROSCIENCE LETTERS

Included Journals: PubMed、SCIE、Scopus

Volume: 501

Issue: 2

Page Number: 112-116

ISSN: 0304-3940

Key Words: Resibufogenin (RBG); Voltage-gated sodium channel (VGSCs); Hippocampal neurons; Whole-cell patch clamp

Abstract: Voltage-gated sodium channels (VGSCs) play important roles in maintaining the excitability of hippocampal neurons. The present study investigated the effects of resibufogenin (RBG, a main component of bufadienolides) on voltage-gated sodium channel currents (I(Na)) in rat hippocampal neurons using whole-cell patch clamp recording. According to the results, RBG activated I(Na) in a concentration-dependent manner. RBG at 1 mu M concentration could alter some channel kinetics of I(Na). such as activation thresholds, steady-state activation and inactivation curves, time constant of recovery, and activity-dependent attenuation of I(Na). RBG influenced peak amplitude, overshoot and half-width of the evoked single action potential, and simultaneously lessened the firing rate of evoked repetitive firing. These findings suggested that I(Na) is probably a target of RBG, which may explain the mechanisms for the pathological effects of RBG on central nervous system. (C) 2011 Elsevier Ireland Ltd. All rights reserved.