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Indexed by:期刊论文

Date of Publication:2009-03-01

Journal:BIOTECHNOLOGY LETTERS

Included Journals:SCIE、EI、PubMed

Volume:31

Issue:3

Page Number:371-376

ISSN No.:0141-5492

Key Words:Aqueous two-phase extraction; 2,3-Butanediol; Cell recycle fermentation; Isopropanol/ammonium sulfate

Abstract:A novel aqueous two-phase system consisted of 2-propanol/ammonium sulfate was used for the extraction of 2,3-butanediol from fermentation broths. The maximum partition coefficient and recovery of 2,3-butanediol reached 9.9 and 93.7%, respectively, and more than 99% of the cells and about 85% of the soluble proteins were removed when 34% (w/w) 2-propanol and 20% (w/w) ammonium sulfate were used. The separated cells could be re-used as inocula for subsequent fermentations. The aqueous two-phase system described in this study may have potential application in the extraction of 2,3-butanediol produced by industrial fermentation processes.