Release Time:2019-03-09  Hits:

Indexed by: Journal Article

Date of Publication: 2009-03-01

Journal: BIOTECHNOLOGY LETTERS

Included Journals: PubMed、EI、SCIE

Volume: 31

Issue: 3

Page Number: 371-376

ISSN: 0141-5492

Key Words: Aqueous two-phase extraction; 2,3-Butanediol; Cell recycle fermentation; Isopropanol/ammonium sulfate

Abstract: A novel aqueous two-phase system consisted of 2-propanol/ammonium sulfate was used for the extraction of 2,3-butanediol from fermentation broths. The maximum partition coefficient and recovery of 2,3-butanediol reached 9.9 and 93.7%, respectively, and more than 99% of the cells and about 85% of the soluble proteins were removed when 34% (w/w) 2-propanol and 20% (w/w) ammonium sulfate were used. The separated cells could be re-used as inocula for subsequent fermentations. The aqueous two-phase system described in this study may have potential application in the extraction of 2,3-butanediol produced by industrial fermentation processes.