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个人信息Personal Information
副教授
硕士生导师
性别：女
毕业院校：大连理工大学
学位：博士
所在单位：生物工程学院
学科：生物化工. 药理学. 神经生物学
办公地点：生物工程学院 323
联系方式：bojiang@dlut.edu.cn 13842650392
电子邮箱：bojiang@dlut.edu.cn
Aqueous two-phase extraction of 2,3-butanediol from fermentation broths by isopropanol/ammonium sulfate system
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论文类型：期刊论文
发表时间：2009-03-01
发表刊物：BIOTECHNOLOGY LETTERS
收录刊物：SCIE、EI、PubMed
卷号：31
期号：3
页面范围：371-376
ISSN号：0141-5492
关键字：Aqueous two-phase extraction; 2,3-Butanediol; Cell recycle fermentation; Isopropanol/ammonium sulfate
摘要：A novel aqueous two-phase system consisted of 2-propanol/ammonium sulfate was used for the extraction of 2,3-butanediol from fermentation broths. The maximum partition coefficient and recovery of 2,3-butanediol reached 9.9 and 93.7%, respectively, and more than 99% of the cells and about 85% of the soluble proteins were removed when 34% (w/w) 2-propanol and 20% (w/w) ammonium sulfate were used. The separated cells could be re-used as inocula for subsequent fermentations. The aqueous two-phase system described in this study may have potential application in the extraction of 2,3-butanediol produced by industrial fermentation processes.