曲景平

个人信息Personal Information

教授

博士生导师

硕士生导师

性别:男

毕业院校:日本东京大学

学位:博士

所在单位:化工学院

电子邮箱:qujp@dlut.edu.cn

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Antibody-Ligand Interactions for Hydrophobic Charge-Induction Chromatography: A Surface Plasmon Resonance Study

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论文类型:期刊论文

发表时间:2015-03-24

发表刊物:LANGMUIR

收录刊物:SCIE、EI、PubMed、Scopus

卷号:31

期号:11

页面范围:3422-3430

ISSN号:0743-7463

摘要:This article describes the use of surface plasmon resonance (SPR) spectroscopy to study antibodyligand interactions for hydrophobic charge-induction chromatography (HCIC) and its versatility in investigating the surface and solution factors affecting the interactions. Two density model surfaces presenting the HCIC ligand (mercapto-ethyl-pyridine, MEP) were prepared on Au using a self-assembly technique. The surface chemistry and structure, ionization, and protein binding of such model surfaces were characterized by X-ray photoelectron spectroscopy (XPS), near-edge X-ray absorption fine structure (NEXAFS), contact-angle titration, and SPR, respectively. The influences of the surface and solution factors, e.g., ligand density, salt concentration, and solution pH, on protein adsorption were determined by SPR. Our results showed that ligand density affects both equilibrium and dynamic aspects of the interactions. Specifically, a dense ligand leads to an increase in binding strength, rapid adsorption, slow desorption, and low specificity. In addition, both hydrophobic interactions and hydrogen bonding contribute significantly to the protein adsorption at neutral pH, while the electrostatic repulsion is overwhelmed under acidic conditions. The hydrophobic interaction at a high concentration of lyotropic salt would cause drastic conformational changes in the adsorbed protein. Combined with the self-assembly technique, SPR proves to be a powerful tool for studying the interactions between an antibody and a chromatographic ligand.