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Indexed by:期刊论文

Date of Publication:2010-07-29

Journal:VETERINARY MICROBIOLOGY

Included Journals:SCIE、PubMed、Scopus

Volume:144

Issue:1-2

Page Number:75-81

ISSN No.:0378-1135

Key Words:TLR; Chicken; Baculovirus; IL-12

Abstract:Our previous work showed that baculovirus (Antheraea pernyi nuclear polyhedrosis virus [ApNPV]) induced a strong innate immunity and protected chicken from a lethal challenge of bronchitis virus. The purpose of present study was to determine the chicken Toll-like receptors (TLRs) used by BV-infected immune cells to induce this immune response. We first investigated the expression of the Toll-like receptor (TLR) genes in resting and BV-infected HD11 (chicken macrophage-like cell line) and DT40 (chicken B cell-like cell line) cells. Expressions of TLRs were detected in both cell types. After BV stimulation, TLR21 was the only upregulated TLR in HD11 cells, and all the TLRs were down-regulated in DT40 cells. Since TLR activation generally leads to cytokine induction, we then determined the expression of IL-12 in the two cell lines following treatment with BV or oligodeoxynucleotides (ODN) containing CpG dinucleotides (CpG-ODN). BV and CpG-ODN treatment induced the expression of IL-12 in HD11 but not in DT40 cells. HD11 cells transfected with siRNA specific for TLR21 significantly diminished BV- and CpG-ODN-induced IL-12 expression. Therefore, BV and CpG-ODN stimulated IL-12 expression involved TLR21 signaling and chicken TLR21 may have similar functions to the mammalian TLR9. (C) 2009 Elsevier B.V. All rights reserved.