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Indexed by:期刊论文

Date of Publication:2009-07-01

Journal:INTERNATIONAL IMMUNOPHARMACOLOGY

Included Journals:SCIE、PubMed、Scopus

Volume:9

Issue:7-8

Page Number:817-823

ISSN No.:1567-5769

Key Words:Baculovirus; Chicken; Signal pathway

Abstract:The budded virus (BV) of Antheraea pernyi is a nuclear polyhedrosis virus (ApNPV), that belongs to the baculovirus family. it is capable of stimulating innate immune response in avian macrophage-like cells (HD11). In this study, we used the expression of proinflammatory cytokines (IFN-gamma and IL-12p40) and production of nitric oxide (NO) as indicators to evaluate the involvement of the signaling cascades in the activation of HD11 cells by BV. Western blot analysis revealed that ERK1/2, p38 MAPK and JNK were phosphorylated followed by activation of p65-NF-kappa B after HD11 cells were challenged with BV. Inhibition of p38 MAPK and NF-kappa B by their respective inhibitors abolished BV-induced expression of IL-12p40, IFN-gamma and production of NO, whereas inhibition of JNK abolished only the induction of cytokines. Additionally, inhibition of ERK1/2 resulted in unexpected increases in both cytokine expression and NO production. Taken together, these findings indicated that in BV-induced HD11 cells, the induction of cytokines was mediated by the p38 MAPK, JNK and NF-kappa B pathways, and NO production was mediated through the p38 MAPK/NF-kappa B pathways. (C) 2009 Elsevier B.V. All rights reserved.