Release Time:2019-03-11  Hits:

Indexed by: Journal Article

Date of Publication: 2018-08-15

Journal: SENSORS AND ACTUATORS B-CHEMICAL

Included Journals: EI、SCIE

Volume: 267

Page Number: 104-110

ISSN: 0925-4005

Key Words: Fluorescent probe; Ratiometric imaging; Confined-space mechanism; Sulfite determination; FRET fabrication

Abstract: A ratiometric fluorescent probe for HSO3- was assembled using a pair of Forster resonance energy transfer (FRET)-matchable fluorescent dyes within a single human serum albumin (HSA). In this work, the micro environment-sensitive dyes B1 and HE were designed and synthesized, wherein B1 located in site II as the FRET donor while HE located in site I as the FRET acceptor and receptor for HSO3-. This B1-HE FRET system exhibited a good efficiency in the sensitive, selective, and ratiometric recognition of HSO3- with a detection limit of 99 nM in aqueous media. Furthermore, this B1-HE system could realize the ratiometric imaging of HSO3- in human breast cancer cells. (C) 2018 Elsevier B.V. All rights reserved.