Release Time:2019-03-12  Hits:

Indexed by: Journal Article

Date of Publication: 2017-10-01

Journal: CHINESE CHEMICAL LETTERS

Included Journals: Scopus、SCIE

Volume: 28

Issue: 10,SI

Page Number: 1991-1993

ISSN: 1001-8417

Key Words: Biomarker; Fluorescent probe; alpha-Ketoglutaricacid; Actue myeloid leukemia (AML); Serum detection

Abstract: alpha-Ketoglutaric acid (alpha-KA) is an important metabolic intermediate in tricarboxylic acid circle in our body. The mutations of isocitrate dehydrogenase-1 (IDH1) and isocitrate dehydrogenase-2 (IDH2), however, will lead to the transformation of alpha-KA into 2-hydroxyglutarate (2-HG), which is confirmed to closely related to actue myeloid leukemia (AML). Therefore it is of great significance to detect alpha-KA level changes in serum. In this paper, a fluorescent "off-on" probe CH for alpha-KA was designed based on naphthalimide fluorophore by introducing a hydrazine group for alpha-KA recognition and a long alkyl amino chain to enhance PET efficiency and water solubility. Cetyltrimethyl ammonium bromide (CTAB) was added toform self-assembly micelles for accelerating the recognition process. CH shows a 28-fold fluorescence enhancement ((I- I-0)/I-0 at 550 nm) over other biological species by optimizing the chemical recognition process of CH with alpha-KA. Significantly, CH was successfully applied for thefluorescence discrimination of all kinds of blood cancer serum samples. This work would provide a potential method that is quick and convenient for sensing alpha-KA and may promote fluorescence detection in clinical diagnosis. (C) 2017 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.