Release Time:2019-03-09  Hits:

Indexed by: Journal Article

Date of Publication: 2014-01-01

Journal: SENSORS AND ACTUATORS B-CHEMICAL

Included Journals: EI、SCIE

Volume: 190

Page Number: 685-693

ISSN: 0925-4005

Key Words: Near-infrared dye; Viscosity probe; Fluorescence ratio; Fluorescence lifetime; Mitochondrial viscosity; Apoptosis

Abstract: Viscosity strongly influences intracellular transportation of mass and signal, and although bulk viscosity can be well measured with macroscopic samples quantities, microscale measurements in live cells still remain a challenge. By introducing a CHO group into the meso-position of the pentamethine chain, a thiazole-Cy5 dye based two-photon fluorescence probe, Mito-V, was developed. It exhibited a selective response to solution viscosity by fluorescence ratio and lifetime methods. More importantly, Mito-V can localize in mitochondria and quantitatively image its viscosity changes by the dual fluorescence mode during the apoptosis. (c) 2013 Elsevier B. V. All rights reserved.