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Indexed by:期刊论文

Date of Publication:2013-12-01

Journal:DYES AND PIGMENTS

Included Journals:SCIE、EI、Scopus

Volume:99

Issue:3

Page Number:620-626

ISSN No.:0143-7208

Key Words:Naphthalimide-rhodamine; FRET; Ratiometric sensing; pH; Fluorescent sensor; Lysosome localization

Abstract:In this work, we presented a naphthalimide-rhodamine based fluorescence resonance energy transfer system (FRET) NR1 as a ratiometric and intracellular pH probe, in which 1,2,3-triazole was identified as an ideal bridge and biocompatibility. It could selectively monitor pH variations in methanol/HEPES solution at room temperature. When the pH changed from 6.20 to 2.00, both the fluorescence intensities at 580 nm and the intensity ratios, R (I-580 nm/I-538 nm) increased, which allowed the detection of pH changes by both normal fluorescence and ratiometric fluorescence methods. The observation is consistent with the increased FRET from the 1,8-naphthalimide (donor) to the ring-opened, colored form of rhodamine (acceptor). Moreover, as NR1 is lysosomal with low cytotoxicity, it will be helpful for investigating the pivotal role of H+ in a biological context, especially in lysosomes through direct intracellular imaging. (C) 2013 Elsevier Ltd. All rights reserved.