Release Time:2019-03-09  Hits:

Indexed by: Journal Article

Date of Publication: 2013-12-01

Journal: DYES AND PIGMENTS

Included Journals: Scopus、EI、SCIE

Volume: 99

Issue: 3

Page Number: 620-626

ISSN: 0143-7208

Key Words: Naphthalimide-rhodamine; FRET; Ratiometric sensing; pH; Fluorescent sensor; Lysosome localization

Abstract: In this work, we presented a naphthalimide-rhodamine based fluorescence resonance energy transfer system (FRET) NR1 as a ratiometric and intracellular pH probe, in which 1,2,3-triazole was identified as an ideal bridge and biocompatibility. It could selectively monitor pH variations in methanol/HEPES solution at room temperature. When the pH changed from 6.20 to 2.00, both the fluorescence intensities at 580 nm and the intensity ratios, R (I-580 nm/I-538 nm) increased, which allowed the detection of pH changes by both normal fluorescence and ratiometric fluorescence methods. The observation is consistent with the increased FRET from the 1,8-naphthalimide (donor) to the ring-opened, colored form of rhodamine (acceptor). Moreover, as NR1 is lysosomal with low cytotoxicity, it will be helpful for investigating the pivotal role of H+ in a biological context, especially in lysosomes through direct intracellular imaging. (C) 2013 Elsevier Ltd. All rights reserved.