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Indexed by:期刊论文

Date of Publication:2010-02-25

Journal:JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY

Included Journals:SCIE

Volume:210

Issue:2-3

Page Number:168-172

ISSN No.:1010-6030

Key Words:Cy5; Cyanine dye; Photophysical property; Photostability; Fluorescent labeling; High performance liquid chromatography

Abstract:Several novel water-soluble asymmetric pentamethine cyanine dyes were synthesized. The maximum absorption and emission wavelengths of the dyes in different solvents were in the range from 647 to 665 nm and exhibited negative solvatochromism with increasing solvent polarity. The fluorescence quantum yields of the dyes were about 0.1 in water, and were obviously higher than those of hydrophobic cyanine dyes. Dyes with N-benzyl groups and N-sulfo-groups displayed greater photostability than dyes with N-carboxypentyl groups in water. The limit of detection of dye 5a for BSA was 1.2 x 10(-8) mol L(-1) by high performance liquid chromatography with fluorescent director about 100-fold lower than that by UV detection (1.0 x 10(-6) mol L(-1)). Therefore, Dye 5a could be used to improve photostability and detection sensitivity in protein analysis. (C) 2010 Elsevier B.V. All rights reserved.