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毛细管电泳测定乳酸发酵液中有机酸

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Indexed by: Journal Article

Date of Publication: 2006-03-30

Journal: 食品与发酵工业

Included Journals: ISTIC、PKU

Volume: 32

Issue: 3

Page Number: 76-78

ISSN: 0253-990X

Key Words: 毛细管电泳;发酵液;有机酸;乳酸

Abstract: 运用毛细管区带电泳模式,在磷酸缓冲液中成功地分离了苹果酸、甲酸、乙酸、丙酸、琥珀酸、酒石酸、柠檬酸、乳酸、草酸9种有机酸.研究了缓冲液浓度、pH值、电泳电压、检测波长等因素对分离的影响,并对实际发酵液进行分析测定,加标回收率为97.5%~107.1%.该方法简便、快速、准确,适于整个发酵过程条件的优化及发酵产物的监控工作.

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