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Indexed by:期刊论文

Date of Publication:2011-02-01

Journal:PROCESS BIOCHEMISTRY

Included Journals:Scopus、SCIE、EI

Volume:46

Issue:2

Page Number:586-591

ISSN No.:1359-5113

Key Words:1,3-Propanediol; Aqueous two-phase extraction; Separation; Dilution crystallization; Fermentation

Abstract:An aqueous two-phase system consisting of methanol and phosphate was used to extract 1,3-propanediol from glycerol-based fermentation broth. Methanol not only extracted 1,3-propanediol effectively from the broth through forming aqueous two-phase system with phosphate, but also allowed the recovery of phosphate by dilution crystallization through adjusting the pH. At 35% (v/v) methanol, saturated concentration of phosphate and a pH of 10.7, the partition coefficient and recovery of 1,3-propanediol reached 38.3 and 98.1%, respectively. The recovery of phosphate in the bottom phase reached 94.7% when 1.5 volume of methanol was added to the salt-rich phase after adjusting the pH to 4.5. At the same time, the main byproduct, 2,3-butandiol was also extracted with high efficiency. In addition, cells and proteins could be simultaneously removed from the fermentation broth, and the removal ratios for cells and proteins reached 99.85% and 92.4%, respectively. Crown Copyright (C) 2010 Published by Elsevier Ltd. All rights reserved.