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发布时间：2019-03-13

论文类型：期刊论文

发表时间：2016-06-01

发表刊物：BIORESOURCE TECHNOLOGY

收录刊物：ESI高被引论文、PubMed、EI、SCIE

卷号：209

页面范围：148-156

ISSN号：0960-8524

关键字：Direct interspecies electron transfer (DIET); Syntrophic metabolism of propionate and butyrate; Up-flow anaerobic sludge blanket (UASB); Biochar

摘要：Promoting direct interspecies electron transfer (DIET) to enhance syntrophic metabolism may be a strategy for accelerating the conversion of organic wastes to methane, but microorganisms capable of metabolizing propionate and butyrate via DIET under methanogenic conditions have yet to be identified. In an attempt to establish methanogenic communities metabolizing propionate or butyrate with DIET, enrichments were initiated with up-flow anaerobic sludge blanket (UASB), similar to those that were previously reported to support communities that metabolized ethanol with DIET that relied on direct biological electrical connections. In the absence of any amendments, microbial communities enriched were dominated by microorganisms closely related to pure cultures that are known to metabolize propionate or butyrate to acetate with production of H-2. When biochar was added to the reactors there was a substantial enrichment on the biochar surface of 16S rRNA gene sequences closely related to Geobacter and Methanosaeta species known to participate in DIET. (C) 2016 Elsevier Ltd. All rights reserved.