个人信息Personal Information
副教授
硕士生导师
性别:女
毕业院校:Regensburg大学
学位:博士
所在单位:化学学院
学科:分析化学
电子邮箱:guohm@dlut.edu.cn
Styryl-BODIPY based red-emitting fluorescent OFF-ON molecular probe for specific detection of cysteine
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论文类型:期刊论文
发表时间:2011-02-15
发表刊物:BIOSENSORS & BIOELECTRONICS
收录刊物:SCIE、EI、PubMed
卷号:26
期号:6
页面范围:3012-3017
ISSN号:0956-5663
关键字:Thiols; Molecular probe; BODIPY; Dark state; Density functional theory; Cysteine
摘要:We have synthesized a styryl boron-dipyrromethene (BODIPY)/2,4-dinitrobenzenesulfonyl (DNBS) dyad based red-emitting molecular probe for specific detection of cysteine among the biological thiols. The probe shows intensive absorption at 556 nm and the probe is non-fluorescent. The DNBS moiety can be cleaved off by thiols, the red emission of the BODIPY fluorophore at 590 nm is switched on, with an emission enhancement of 46-fold. The probe shows good specificity toward cysteine over other biological molecules, such as glutathione and amino acids. The emission of the probe is pH-independent in the physiological pH range. The probe is used for fluorescent imaging of cellular thiols. Theoretical calculations based on density functional theory (DFT) were used to elucidate the fluorescence sensing mechanism of the probe, which indicate a dark excited state (S-1) for the probe but an emissive excited state (S-1) for the cleaved product (i.e. the fluorophore). (C) 2010 Elsevier B.V. All rights reserved.