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Indexed by:Journal Papers

Date of Publication:2015-07-01

Journal:JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A

Included Journals:SCIE、EI、PubMed、Scopus

Volume:103

Issue:7

Page Number:2333-2343

ISSN No.:1549-3296

Key Words:microcapsule; transplantation; cell adhesion; peritoneal cavity; inflammatory response

Abstract:Cell microencapsulation technology is a potential alternative therapy, but cell overgrowth and adhesion on the microcapsules after transplantation shortens their time of therapeutic efficacy. Inflammatory cells were the main cells that adhered to the microcapsules, so understanding the body's inflammatory processes would help to better identify the mechanisms of cell adhesion to the outer surface of the microcapsules. Our study measured the inflammatory cells and the cytokines and characterized the associated changes in the alginate-chitosan-alginate (ACA) microcapsules 1, 7, 14, and 28 days after implantation in the peritoneal cavity. Then the relationship between the inflammatory response and cell adhesion on the microcapsules was evaluated by multiple regression analysis. The results showed that the microcapsules did not evoke a systemic inflammatory response, but initiated a local inflammatory response in the peritoneal cavity. Furthermore, the correlation analysis showed that the level of cell adhesion on the microcapsules was related to the number of lymphocytes and macrophages, and the amount of IL-6, IL-10, and MCP-1 in the peritoneal cavity. Our results may provide a foundation for reducing the immune response to these microcapsules, prolonging graft survival and improving the efficacy of these treatments. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103: 2333-2343, 2015.