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论文类型：期刊论文

发表时间：2015-07-01

发表刊物：JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A

收录刊物：SCIE、EI、PubMed、Scopus

卷号：103

期号：7

页面范围：2333-2343

ISSN号：1549-3296

关键字：microcapsule; transplantation; cell adhesion; peritoneal cavity; inflammatory response

摘要：Cell microencapsulation technology is a potential alternative therapy, but cell overgrowth and adhesion on the microcapsules after transplantation shortens their time of therapeutic efficacy. Inflammatory cells were the main cells that adhered to the microcapsules, so understanding the body's inflammatory processes would help to better identify the mechanisms of cell adhesion to the outer surface of the microcapsules. Our study measured the inflammatory cells and the cytokines and characterized the associated changes in the alginate-chitosan-alginate (ACA) microcapsules 1, 7, 14, and 28 days after implantation in the peritoneal cavity. Then the relationship between the inflammatory response and cell adhesion on the microcapsules was evaluated by multiple regression analysis. The results showed that the microcapsules did not evoke a systemic inflammatory response, but initiated a local inflammatory response in the peritoneal cavity. Furthermore, the correlation analysis showed that the level of cell adhesion on the microcapsules was related to the number of lymphocytes and macrophages, and the amount of IL-6, IL-10, and MCP-1 in the peritoneal cavity. Our results may provide a foundation for reducing the immune response to these microcapsules, prolonging graft survival and improving the efficacy of these treatments. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103: 2333-2343, 2015.