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Indexed by:会议论文

Date of Publication:2011-01-22

Included Journals:EI、CPCI-S、SCIE、Scopus

Volume:7909

Key Words:Ag/SiO2 nanocomposites; metal-enhanced fluorescence; time-gated bioimaging; Eu chelate; single particle detection

Abstract:We report silver nanostructure-enhanced fluorescence of a europium (Eu) chelate, BHHCT-Eu-DPBT, which was covalently bound in Ag/SiO2 nanocomposites. This design enhances the europium signal intensity by more than one order of magnitude, and accelerates the decay time from 0.3 ms down to 60 microseconds, at low excitation conditions. These nanocomposites were bright enough to be observed in time-gated fluorescence microscopy under 365 nm LED excitation. The increased brightness and reduced lifetime of such fluorescent core-shell nanocomposites will enhance their applicability for ultrasensitive bioassays and bioimaging, especially with time-gating.