Release Time:2019-03-09  Hits:

Indexed by: Journal Article

Date of Publication: 2010-01-01

Journal: JOURNAL OF FLUORESCENCE

Included Journals: Scopus、PubMed、EI、SCIE

Volume: 20

Issue: 1

Page Number: 321-328

ISSN: 1053-0509

Key Words: Lanthanide; Multicolor luminescent nanoparticle; Biolabeling; Time-resolved luminescence bioassay

Abstract: Because highly luminescent lanthanide compounds are limited to Eu(3+) and Tb(3+) compounds with red (Eu, similar to 615 nm) and green (Tb, similar to 545 nm) emission colors, the development and application of time-resolved luminescence bioassay technique using lanthanide-based multicolor luminescent biolabels have rarely been investigated. In this work, a series of lanthanide complexes covalently bound silica nanoparticles with an excitation maximum wavelength at 335 nm and red, orange, yellow and green emission colors has been prepared by co-binding different molar ratios of luminescent Eu(3+)-Tb(3+) complexes with a ligand N,N,N(1),N(1)-(4'-phenyl-2,2':6',2''-terpyridine-6,6''-diyl)bis(methylenenitrilo) tetrakis (acetic acid) inside the silica nanoparticles. The nanoparticles characterized by transmission electron microscopy and luminescence spectroscopy methods were used for streptavidin labeling, and time-resolved fluoroimmunoassay (TR-FIA) of human prostate-specific antigen (PSA) as well as time-resolved luminescence imaging detection of an environmental pathogen, Giardia lamblia. The results demonstrated the utility of the new multicolor luminescent lanthanide nanoparticles for time-resolved luminescence bioassays.