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论文类型：期刊论文

发表时间：2016-07-01

发表刊物：JOURNAL OF LUMINESCENCE

收录刊物：SCIE、EI

卷号：175

页面范围：122-128

ISSN号：0022-2313

关键字：NBD; Chemosensing ensemble; Thiols; Detection; Fluorescence imaging

摘要：Based on an aldazine-copper chemosensing ensemble (NP-Cu2+), a new fluorescence chemosensor for the detection of biothiols (Cys, Hcy and GSH) was designed and synthesized. In aqueous solution, the ligand NP exhibited high selectivity toward Cu2+ ions by forming a 2:1 complex, accompanied with a dramatic fluorescence quenching and a notable bathochromic-shift of the absorbance band. Due to the high affinity of thiols and copper, the specific interaction of thiols (Cys, Hcy and GSH) with NP-Cu2+ ensemble led to the liberation of the NP. As the result, recovery of fluorescence and UV-vis absorbance was observed. The detection limits of NP-Cu2+ to Cys, Hcy and GSH were estimated to be 1.5 mu M, 1.8 mu M and 2.2 mu M, respectively. The fluorescence "OFF-ON" circle can be repeated to a minimum of 5 times by the alternative addition of thiols and Cu2+, implying that NP-Cu2+ is a recyclable chemosensor for thiols. Results of fluorescence microscopy imaging suggested that NP-Cu2+ has potential to be used as a powerful tool for the detection of intracellular thiols. (C) 2016 Elsevier B.V. All rights reserved.