何成

个人信息Personal Information

教授

博士生导师

硕士生导师

性别:男

毕业院校:南京大学

学位:博士

所在单位:化工学院

电子邮箱:hecheng@dlut.edu.cn

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Fluorimetric and colorimetric detection of nitric oxide in living cells by rhodamine derivatives assisted by Cu2+

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论文类型:期刊论文

发表时间:2013-01-14

发表刊物:TETRAHEDRON

收录刊物:SCIE、Scopus

卷号:69

期号:2

页面范围:636-641

ISSN号:0040-4020

关键字:Nitric oxide; Rhodamine; Cu2+; Fluorescence imaging

摘要:Two rhodamine-based NO probes were designed and synthesized. Both RB-TP and RB-Py can detect NO by spectroscopic and 'naked-eye' methods in HEPES buffer assisted by Cu2+. The detection limits for NO were 5 nM and 20 nM, respectively. Other biologically relevant reactive species, such as NO2-, NO3-, OONO-, H2O2, O-1(2), and ClO- did not give any obvious interferences. RB-TP and RB-Py have been also applied to detect the intracellular NO by fluorescence imaging method. Crown Copyright (c) 2012 Published by Elsevier Ltd. All rights reserved.