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Indexed by:期刊论文

Date of Publication:2012-02-01

Journal:COLLOIDS AND SURFACES B-BIOINTERFACES

Included Journals:SCIE、EI、PubMed、Scopus

Volume:90

Issue:1

Page Number:248-253

ISSN No.:0927-7765

Key Words:Adsorbent; Bilirubin; Cyclodextrin; Dialysis; Docking

Abstract:In this study, we explored the use of alpha-, beta- or gamma-cyclodextrin (CD)-grafted polyethyleneimine (PEI) as water-soluble adsorbent for removing excess plasma bilirubin. To evaluate the bilirubin-binding capacity of these adsorbents, bovine serum albumin (BSA) solution or plasma with high level of bilirubin were dialyzed against CD-PEI-spiked dialysate. In BSA solution with an initial biliurbin concentration of 171.5 mg/L, alpha-CD-PEI, beta-CD-PEI and gamma-CD-PEI achieved adsorption capacities of 2.5, 5.8 and 3.8 mg/g, respectively. In a plasma dialysis system, 45.6% of bilirubin (260 mg/L) was removed from 200 mL plasma by 1 L dialysate spiked with 10 mg/mL beta-CD-PEI, which was significantly higher than that removed by the same volume of BSA-spiked dialysate (P < 0.05), demonstrating the strong bilirubin-binding ability of beta-CD-PEI. The key feature of bilirubin adsorption was related to the CD functional group, not the PEI matrix. Subsequent molecular docking study indicated that the size of CD cavity could affect the affinity energy of CD-bilirubin complex. The cavity of beta-CD was most suitable for accommodating the pyrrole rings of bilirubin. The inclusion complex of bilirubin and beta-CD in the molar ratio of 1:2 was more logical in terms of affinity energy. All the results demonstrated the potential of beta-CD-PEI (water-soluble adsorbent) as an effective agent for removing of bilirubin from plasma in dialysis system. (C) 2011 Elsevier B.V. All rights reserved.