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论文类型：期刊论文

发表时间：2019-02-01

发表刊物：JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY

收录刊物：SCIE、Scopus

卷号：370

页面范围：12-17

ISSN号：1010-6030

关键字：NIR emission; Hydrogen peroxide; Large Stokes shift; Cell imaging

摘要：Hydrogen peroxide (11202), regarded as the byproduct of aerobic metabolism via the electron transport chain, plays a significant role in a vast array of physiological and pathological process in the organisms. Therefore, it is of great importance to develop efficient analytical approach for monitoring the H2O2 level in living systems. Herein, in this work, a NIR fluorescent probe named NDCM-HP was developed for the highly selective detection of H2O2 by introducing boronate group as the recognition unit into the 2-(3-(4-hydroxystyryl)-5,5-dimethylcyclohex-2-enylidene) malononitrile (NDCM-OH) fluorophore which is featured by strong NIR emission, large Stokes shift, and good photostability. As a matter of fact, NDCM-HP demonstrated excellent sensing performance toward H2O2 in solution samples such as high sensitivity with about 127-fold emission enhancement, low detection limit of 70.189 nM, and good selectivity over biologically related species. Furthermore, it could be employed for detecting endogenous H2O2 fluctuation in living cells induced by different stimulators such as Phorbol myristate acetate (PMA), N-Acetylcysteine (NAC), indicating its great potential in biological application.