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发布时间：2019-03-09

论文类型：期刊论文

发表时间：2012-03-01

发表刊物：MICROFLUIDICS AND NANOFLUIDICS

收录刊物：EI、SCIE

卷号：12

期号：5

页面范围：829-834

ISSN号：1613-4982

关键字：Deposited microbead plug (DMBP); Whole-blood immunoassay; Capillary force; Microfluidics

摘要：We present a deposited microbead plug (DMBP)-based microfluidic chip capable of performing plasma extraction and on-chip immunoassay. The DMBP used as a porous blood filter provides pure blood plasma without the contamination of blood cells or beads. Capillary-driven flow eliminates the requirement of external pumps. The human IgG and goat anti-human IgG sample-to-answer assay was performed in this chip within 600 s using only a 10 mu l whole-blood sample. This easy-to-use, rapid, inexpensive, and disposable DMBP-based chip holds a great promise for point-of-care application.