A novel fluorescent probe based on naphthalimide for imaging nitroreductase (NTR) in bacteria and cells
Release Time:2021-04-01 Hits:
Indexed by: Journal Papers
Date of Publication: 2021-04-01
Journal: BIOORGANIC & MEDICINAL CHEMISTRY
Volume: 28
Issue: 3
Page Number: 115280
ISSN: 0968-0896
Key Words: Nitroreductase (NTR); 1,8-Naphthalimide; Escherichia coli (E. coli); Staphylococcus aureus. (S. aureus)
Abstract: A nitroreductase (NTR) responsive fluorescent probe, Na-NO2, comprising p-nitrobenzyl as the unique recognition group and 1,8-naphthalimide as fluorophore, was synthesized. Na-NO2 showed remarkable fluorescence "turn-on" signal in the presence of NTR under DMSO/H2O (1:19, v/v) buffered with PBS (pH = 7) solution in the presence of NADH (300 mu M). Furthermore, the probe has a low detection limit down to 3.4 ng/mL and it is very sensitive towards the NTR in Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), normal and tumor cells such as HL-7702, HepG-2 and MCF-7.