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Interaction study on DNA, single-wall carbon nanotubes and acridine orange

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Indexed by:期刊论文

Date of Publication:2012-06-25

Journal:MATERIALS SCIENCE AND ENGINEERING B-ADVANCED FUNCTIONAL SOLID-STATE MATERIALS

Included Journals:SCIE、EI

Volume:177

Issue:11

Page Number:887-891

ISSN No.:0921-5107

Key Words:Single-wall carbon nanotubes (SWCNTs); Acridine orange (AO); Hybrid; DNA; Fluorescence detection

Abstract:The fluorescence of acridine orange (AO) can be quenched significantly with addition of the single-wall carbon nanotubes (SWCNTs) solution, due to the formation of a hybrid complex between AO and SWCNTs. A fluorescence enhancement of approximately 18 x can be observed after the addition of certain amount of DNA into the above mentioned solution. The fluorescence increase was linearly proportional to the amount of DNA added in the concentration range of 0-50.75 mu M, and the DNA detection limit was down to 8.56 x 10(-8) M. This method can be used to detect DNA in vitro. (C) 2012 Elsevier B.V. All rights reserved.

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