Release Time:2019-03-09  Hits:

Indexed by: Journal Article

Date of Publication: 2010-02-01

Journal: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Included Journals: Scopus、PubMed、EI、SCIE

Volume: 160

Issue: 3

Page Number: 773-779

ISSN: 0273-2289

Key Words: Analgesic action; Gln49 phospholipase A(2); Neurotoxin; Patch clamp; Ion channel; Gloydius ussurensis

Abstract: Gln49-PLA(2) is a phospholipase A(2) isolated from the Gloydius ussurensis snake venom. In this paper, we studied its effect on the function of neural conduction. Electrophysiological studies demonstrated that Gln49-PLA(2) reduced the amplitude of the action potential and the velocity of nerve conduction on isolated mouse sciatic nerve. Patch clamp recordings confirmed that Gln49-PLA(2) significantly decreased neural excitability by the potentiation of sodium channels and the blockade of potassium channels in nerve terminal. In freshly isolated hippocampal pyramidal neurons, 54.25% of potassium current was inhibited by 20 mu g/ml Gln49-PLA(2). However, sodium current was potentiated by 158.99% under the same condition. These findings demonstrate that the effect of Gln49-PLA(2) on ion channels is the main mechanism of analgesic action.