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Indexed by:期刊论文

Date of Publication:2018-01-01

Journal:Phytomedicine : international journal of phytotherapy and phytopharmacology

Included Journals:SCIE、PubMed、Scopus

Volume:38

Page Number:24-34

ISSN No.:1618-095X

Key Words:Mechanism,Oroxin A,PPARγ,Prediabetes,Type 2 diabetes

Abstract:BACKGROUND: Oroxylum indicum (L.) Kurz (Bignoniaceae) has been widely used for the treatment of respiratory infections and gastrointestinal disorders. Our previous study showed that an ethanol-water O. indicum seed extract (OISE), when combined with acarbose, reduced the risk of diabetes by 75% and effectively prevented the associated complications. Oroxin A, a major component of OISE, can activate PPARgamma and inhibit alpha-glucosidase and it represents a promising candidate for diabetes intervention.; PURPOSE: The aim of this study is to investigate the effect of oroxin A from O. indicum on the progression of prediabetes to diabetes and the underlying mechanisms in streptozotocin and high-fat-diet induced prediabetic mice.; METHODS: Oroxin A was purified from OISE and its PPARgamma transcriptional activation was determined in vitro and in vivo. The prediabetic mice were established by high-fat diet and streptozotocin, which was followed by treatment with oroxin A. The effect of oroxin A was determined by analysis of the lipid profiles, oxidative stress, hepatic function and histology. The mechanism of oroxin A was also investigated.; RESULTS: Oroxin A is a compound with low toxicity that has reduced the relative risk of progression from prediabetes to diabetes by 66.7% without inducing weight gain or hepatotoxicity. Oroxin A also improved the complications of prediabetes, such as lipid metabolism dysfunction and liver injury. Results of mechanism studies suggested that oroxin A is a partial PPARgamma agonist that can activate PPARgamma transcriptional activation in vitro and in vivo. Oroxin A also exhibited an inhibitory activity against alpha-glucosidase and an antioxidant capacity.; CONCLUSION: Oroxin A prevents the progression from prediabetes to diabetes through a multi-pathway intervention mechanism. Copyright © 2017 Elsevier GmbH. All rights reserved.