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Indexed by:期刊论文

Date of Publication:2017-07-01

Journal:BIOMEDICINE & PHARMACOTHERAPY

Included Journals:SCIE、PubMed、Scopus

Volume:91

Page Number:890-898

ISSN No.:0753-3322

Key Words:Oroxylum indicum; Acarbose; Synergism; Postprandial blood glucose; alpha-Glucosidase; Baicalein

Abstract:The combined effect of Oroxylum indicum seed extracts (OISE) or major flavonoids from OISE and acarbose on reducing postprandial blood glucose (PBG) levels was investigated in vitro and in vivo. In vitro, the IC50 values of OISE and baicalein against alpha-glucosidase were 43.4 + 0.731 mu g mL(-1) and 25.9 + 0.412 mu g mL(-1) respectively. A combination of acarbose with OISE or baicalein synergistically inhibited rat intestinal alpha-glucosidase. The combination index (CI) values for acarbose with OISE ranged from 0.33 to 0.75, suggesting a synergistic but not additive effect. OISE was determined to be a non-competitive inhibitor of maltose-hydrolyzing activity. In vivo, OISE were administered to normoglycemic and diabetic mice, either alone or in combination with acarbose. At doses between 50 and 200 mg kg(-1), OISE enhanced the efficacy of acarbose by up to 5-fold. These results demonstrated that OISE enhances the efficacy of acarbose in vivo, and that the combination of OISE and acarbose displayed a synergistic effect in vitro. Therefore, OISE can be used to design dietary supplements to treat diabetes. (C) 2017 Published by Elsevier Masson SAS.