期刊论文

Shang, Fei

Quan, CS; Xu, YB (reprint author), Dalian Minzu Univ, Coll Life Sci, Dept Bioengn, Dalian 116600, Liaoning, Peoples R China.; Nam, KH (reprint author), Korea Univ, Inst Life Sci & Nat Resources, Seoul 02841, South Korea.

Quan, Chunshan,Xu, Yongbin,Chen, Jinli,Wang, Lulu,Jin, Liming,Zou, Linhai,Bu, Tingting,Dong, Yuesheng,Ha, Nam-Chul,Nam, Ki Hyun

2018-09-18

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

PubMed、SCIE

J

503

4

2906-2911

0006-291X

Bacillus subtilis; Nicotinamidase/pyrazinamidase; Nicotinamide (NAM); Pyrazinamide (PZA); PncA

The nicotinamidase/pyrazinamidase PncA is a member of a large family of hydrolase enzymes that catalyze the deamination of nicotinamide to nicotinic acid. PncA also functions as a pyrazinamidase in a wide variety of eubacteria and is an essential coenzyme in many cellular redox reactions in living systems. We report the crystal structure of substrate-free PncA from Bacillus subtilis (BsPncA) at 2.0 angstrom resolution to improve our understanding of the PncA family. The structure of BsPncA consists of an alpha/beta domain and a subdomain. The subdomain of BsPncA has a different conformation than that of PncA enzymes from other organisms. The B-factor analysis revealed a rigid structure of the alpha/beta domain, while the subdomain is highly flexible. Both dimers and tetramers were observed in BsPncA protein crystals, but only dimers were observed in solution. Our results provide useful information that will further enhance our understanding of the molecular functions of PncA family members. (C) 2018 Published by Elsevier Inc.