Indexed by:期刊论文
Date of Publication:2016-02-04
Journal:ANALYTICA CHIMICA ACTA
Included Journals:SCIE、EI、PubMed
Volume:906
Page Number:80-88
ISSN No.:0003-2670
Key Words:Silver nanoclusters and graphene oxide nanocomposites; Signal amplification; Enzyme-free; Electrochemical immunosensors; Gold nanoparticle and chitosan matrix
Abstract:Silver nanoclusters and graphene oxide nanocomposite (AgNCs/GRO) is synthesized and functionalized with detection antibody for highly sensitive electrochemical sensing of carcinoembryonic antigen (CEA), a model tumor marker involved in many cancers. AgNCs with large surface area and abundant amount of low-coordinated sites are synthesized with DNA as template and exhibit high catalytic activity towards the electrochemical reduction of H2O2. GRO is employed to assemble with AgNCs because it has large specific surface area, super electronic conductivity and strong pi-pi stacking interaction with the hydrophobic bases of DNA, which can further improve the catalytic ability of the AgNCs. Using AgNCs/GRO as signal amplification tag, an enzyme-free electrochemical immunosensing protocol is designed for the highly sensitive detection of CEA on the capture antibody functionalized immunosensing interface. Under optimal conditions, the designed immunosensor exhibits a wide linear range from 0.1 pg mL(-1) to 100 ng mL(-1) and a low limit of detection of 0.037 pg mL(-1). Practical sample analysis reveals the sensor has good accuracy and reproducibility, indicating the great application prospective of the AgNCs/GRO in fabricating highly sensitive immunosensors, which can be extended to the detection of various kinds of low abundance disease related proteins. (C) 2015 Elsevier B.V. All rights reserved.
Professor
Supervisor of Doctorate Candidates
Supervisor of Master's Candidates
Main positions:化学学院副院长
Other Post:辽宁省教学名师,宝钢优秀教师特等奖提名奖,中国分析测试学会青年学术委员会委员
Gender:Female
Alma Mater:南京大学
Degree:Doctoral Degree
School/Department:化学学院
Discipline:Analytical Chemistry. Biochemistry and Molecular Biolog
Business Address:化工综合楼C305
Open time:..
The Last Update Time:..