Indexed by:期刊论文
Date of Publication:2014-04-01
Journal:ANALYTICAL CHEMISTRY
Included Journals:SCIE、EI、Scopus
Volume:86
Issue:7
Page Number:3461-3467
ISSN No.:0003-2700
Abstract:Single nucleotide polymorphism (SNP) detection is important for early diagnosis, clinical prognostics, and disease prevention, and a rapid and sensitive low-cost SNP detection assay would be valuable for resource-limited clinical settings. We present a simple platform that enables sensitive, naked-eye detection of SNPs with minimal reagent and equipment requirements at room temperature within 15 min. SNP detection is performed in a single tube with one set of DNA probe-modified gold nanoparticles (AuNPs), a single exonuclease (Exo III), and the target in question. Exo III's apurinic endonucleolytic activity differentially processes hybrid duplexes between the AuNP-bound probe and DNA targets that are perfectly matched or contain a single-base mismatch. For perfectly matched targets, Exo III's exonuclease activity facilitates a process of target recycling that rapidly shears DNA probes from the particles, generating an AuNP aggregation-induced color change, whereas no such change occurs for mismatched targets. This color change is easily observed with as little as 2 nM of target, 100-fold lower than the target concentration required for reliable naked eye observation with unmodified AuNPs in well-optimized reaction conditions. We further demonstrate that this system can effectively discriminate a range of different mismatches.
Professor
Supervisor of Doctorate Candidates
Supervisor of Master's Candidates
Main positions:化学学院副院长
Other Post:辽宁省教学名师,宝钢优秀教师特等奖提名奖,中国分析测试学会青年学术委员会委员
Gender:Female
Alma Mater:南京大学
Degree:Doctoral Degree
School/Department:化学学院
Discipline:Analytical Chemistry. Biochemistry and Molecular Biolog
Business Address:化工综合楼C305
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