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论文类型：期刊论文

发表时间：2020-03-02

发表刊物：The Analyst

收录刊物：PubMed

卷号：145

期号：5

页面范围：1933-1942

ISSN号：1364-5528

摘要：A simple and easy-operation electrode modification strategy was proposed using Cu-MOF/GO nanohybrids for physiologists and pathologists for the feasible and reliable simultaneous electrochemical detections of DNA bases, namely guanine and adenine. The nanohybrids were prepared via a simple ultrasonic method and were employed for the fabrication of a sensing interface. SEM, TEM, XRD, FT-IR, and electrochemical characterizations were used to characterize the general morphology and structure of the nonohybrids. The proposed Cu-MOF/ERGO/GCE exhibited ultra-stable and high-sensitivity performance in the simultaneous electrochemical detection of guanine and adenine. The recorded DPV curves revealed a linear increase in the faradaic signals with increase in the concentrations of guanine and adenine in the range of 0.02-10 µM and 20-100 µM for guanine, and 0.005-20 µM and 40-200 µM for adenine. The relative standard deviation of guanine and adenine for 50 consecutive detections is 1.37% and 1.92%, respectively. It was proved that the proposed Cu-MOF/ERGO/GCE can be performed for the detection of guanine and adenine in real samples, such as Herring sperm DNA, and satisfactory results were obtained. This strategy does not require complicated modification procedures, professional modification techniques, or sophisticated instruments, but it can provide a highly sensitive and stable detection method, which is expected to expand and deepen the applications of electrochemical detection in life science research.