个人信息Personal Information
教授
博士生导师
硕士生导师
主要任职:化学学院副院长
其他任职:辽宁省教学名师,宝钢优秀教师特等奖提名奖,中国分析测试学会青年学术委员会委员
性别:女
毕业院校:南京大学
学位:博士
所在单位:化学学院
学科:分析化学. 生物化学与分子生物学
办公地点:化工综合楼C305
电子邮箱:wushuo@dlut.edu.cn
A Label-Free Aptamer-Fluorophore Assembly for Rapid and Specific Detection of Cocaine in Biofluids
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论文类型:期刊论文
发表时间:2014-11-18
发表刊物:ANALYTICAL CHEMISTRY
收录刊物:SCIE、EI、Scopus
卷号:86
期号:22
页面范围:11100-11106
ISSN号:0003-2700
摘要:We report a rapid and specific aptamer-based method for one-step cocaine detection with minimal reagent requirements. The feasibility of aptamer-based detection has been demonstrated with sensors that operate via target-induced conformational change mechanisms, but these have generally exhibited limited target sensitivity. We have discovered that the cocaine-binding aptamer MNS-4.1 can also bind the fluorescent molecule 2-amino-5,6,7-trimethyl-1,8-naphthyridine (ATMND) and thereby quench its fluorescence. We subsequently introduced sequence changes into MNS-4.1 to engineer a new cocaine-binding aptamer (38-GC) that exhibits higher affinity to both ligands, with reduced background signal and increased signal gain. Using this aptamer, we have developed a new sensor platform that relies on the cocaine-mediated displacement of ATMND from 38-GC as a result of competitive binding. We demonstrate that our sensor can detect cocaine within seconds at concentrations as low as 200 nM, which is 50-fold lower than existing assays based on target-induced conformational change. More importantly, our assay achieves successful cocaine detection in body fluids, with a limit of detection of 10.4, 18.4, and 36 mu M in undiluted saliva, urine, and serum samples, respectively.