Release Time:2019-03-13  Hits:

Indexed by: Journal Article

Date of Publication: 2016-07-01

Journal: INTERNATIONAL IMMUNOPHARMACOLOGY

Included Journals: Scopus、PubMed、SCIE

Volume: 36

Page Number: 305-314

ISSN: 1567-5769

Key Words: Egg yolk antibodies; IgY; Salmonella typhimurium; Mucosal immune response; Intestinal inflammation; Gut-associated lymphoid tissue

Abstract: This study determined the effects of chicken egg yolk antibodies (IgY) on immune responses in the intestinal mucosal of mice infected with Salmonella typhimurium. Sixty, 28-day-old mice were divided into 4 groups and treated with streptomycin or sterile water for 2 days followed by 1 day without treatment. The control group was unchallenged whereas the mice in the other three groups were treated twice with 10(9) CFU mL(-1) S. typhimurium. For the next 3 days, control mice continued to receive no treatment whereas the mice in the remaining three groups were orally administered with 20 mg mL(-1) of specific IgY, 20 mg mL(-1) of nonspecific IgY or PBS. S. typhimurium activated gut-associated lymphoid tissue, increasing the release of IFN-gamma and TNF-alpha in the mucosa and increased the number of activated T-lymphocytes and cytotoxic T-gamma delta. Specific IgY attenuated the increase in IFN-gamma and TNF-alpha and the decrease in IL-10. S. typhimurium induced mobilization of CD8(+) and CD8(+) TCR gamma delta T cells in the epithelium and CD4(+) and CD8(+) T cells in the lamina propria reflecting an inflammatory process that was attenuated by IgY. These results suggest that specific IgY modulates intestinal mucosal immune responses during a S. typhimurium infection. (C) 2016 Elsevier B.V. All rights reserved.