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Indexed by:期刊论文

Date of Publication:2012-07-26

Journal:AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH

Included Journals:SCIE

Volume:6

Issue:28

Page Number:5789-5795

ISSN No.:1996-0808

Key Words:Polymerase chain reaction-based denaturing gradient gel electrophoresis (PCR-DGGE); 16S rRNA; halitosis; microbial profiles

Abstract:The purpose of this study was to compare the microbial profiles on the tongue dorsa of healthy subjects and subjects with halitosis using polymerase chain reaction-based denaturing gradient gel electrophoresis (PCR-DGGE). Tongue dorsum scrapings from five healthy subjects and six subjects with halitosis were analyzed by PCR-DGGE using primers specific for the V6 to V8 region of the eubacterial 16S rRNA gene. A dendrogram was derived from the PCR-DGGE fingerprints and the prominent DGGE bands of interest were identified through DNA sequencing. The similarity of the PCR-DGGE fingerprints was determined using the unweighted pair group method with an arithmetic mean dendrogram derived using Dice's Coefficient of Similarity. The sequence of PCR amplicons indicated that the microbial species most associated with halitosis were Haemophilus parainfluenzae and a phylotype of Lachnospiraceae (Lachnospiraceae genomosp. C1), whereas Streptococcus salivarius subsp. salivarius, Neisseria mucosa and Neisseria cinerea were species that did not appear to be associated with halitosis and are likely part of the healthy tongue flora. These results suggest that the presence of H. parainfluenzae and Lachnospiraceae genomosp. C1 may be associated with a shift in the balance of oral microbes in subjects with halitosis.