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Indexed by:期刊论文

Date of Publication:2011-12-01

Journal:JOURNAL OF APPLIED MICROBIOLOGY

Included Journals:Scopus、SCIE

Volume:111

Issue:6

Page Number:1524-1532

ISSN No.:1364-5072

Key Words:egg yolk immunoglobulin; endotoxemia; interleukin-10; lipopolysaccharide; tumour necrosis factor-alpha

Abstract:Aims: To estimate the efficacy of specific egg yolk immunoglobulin (IgY) for the treatment of lipopolysaccharide (LPS)-induced endotoxemia using a mouse model.
   Methods and Results: Specific IgY was obtained from the yolk of hens immunized with formaldehyde-killed Escherichia coli O111 and showed a high binding activity to LPS when subjected to an ELISA. Endotoxemia was induced in mice by intraperitoneal injection of LPS at a dose of 20 mg kg(-1) for measuring survival rate and 10 mg kg(-1) for cytokine measurement. The survival rate of mice treated with 200 mg kg(-1) specific IgY or 5 mg kg(-1) dexamethasone was 70% while none of the mice in the normal saline-treated group survived more than 7 days. Specific IgY significantly (P < 0 05) decreased tumour necrosis factor-alpha (TNF-alpha) level and increased interleukin-10 (IL-10) level in the serum of endotoxemia mice. Specific IgY had less of an effect on TNF-alpha than dexamthasone, while its effect on increasing IL-10 was stronger than dexamethasone. Haematoxylin and eosin-stained sections indicated that IgY attenuated the damage to the lung and liver observed in mice with endotoxemia.
   Conclusions: The specific IgY increased the survival rate of mice with endotoxemia induced by LPS, down-regulated TNF-alpha and up-regulated IL-10 in serum and attenuated the extent of damage to the lung and liver.
   Significance and Impact of the Study: The specific IgY has potential for the treatment of LPS-induced endotoxemia.