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Indexed by:期刊论文

Date of Publication:2008-04-01

Journal:COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY

Included Journals:SCIE、PubMed

Volume:147

Issue:3

Page Number:293-298

ISSN No.:1532-0456

Key Words:Apostichopus japonicus (Selenka); Astragalus membranaceus; phagocytosis; superoxide anion (O-2(-)) production; phagocytic amoebocytes; temperature

Abstract:The potential immunostimulatory effects of Astralagus membranaceus polysaccharides (APS) on sea cucumber, Apostichopus japonicus (Selenka), were investigated in vitro. Phagocytosis and superoxide anion (O-2(-)) production by phagocytic amoebocytes (PA) from A. japonicus coelomic fluid were measured during incubation at 18 degrees C, 22 degrees C, or 25 degrees C with APS at 0, 10, 20, or 40 mu g mL(-1) (n=3). Phagocytic activity against yeast cells was quantified by direct visualization, and O-2(-) production by nitroblue tetrazolium (NBT) reduction assay. Compared with controls, including APS at 20 mu g mL(-1) significantly increased (P< 0.05) the percentage of phagocytic capacity (PC) and phagocytic index (PI) at 18 degrees C and 22 degrees C, but no significant enhancement was observed at 25 C. In contrast, the coelmocytes of A. japonicus can have an obvious generation of O-2(-) after the stimulation. The concentration of 20 mu g mL(-1) APS resulted in a significant increase in nitroblue tetrazolium (NBT) positive cells (P<0.05) at different temperature and even 10 mu g mL(-1) APS could increase O-2(-) generation significantly at 18 degrees C and 22 degrees C. Both phagocytosing and O-2(-) production increased with the increase of APS concentration from 0 to 20 mu g mL(-1) at different temperature, and when APS at 40 mu g mL(-1), they were decreased. It suggested that immunocytes activity in A. japonicus decreased with the temperature increasing from 18 C to 25 degrees C, and APS could be an effective immunostimulant to enhance phagocytic activity and O-2(-) production. (c) 2007 Elsevier Inc. All rights reserved.