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抗奶牛乳腺炎多价卵黄抗体的制备及含量测定

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Indexed by:期刊论文

Date of Publication:2009-03-20

Journal:中国畜牧兽医

Included Journals:PKU、ISTIC

Volume:36

Issue:3

Page Number:134-136

ISSN No.:1671-7236

Key Words:卵黄抗体;奶牛乳腺炎;金黄色葡萄球菌;无乳链球菌;大肠杆菌

Abstract:本研究的目的是制备抗奶牛乳腺炎主要致病菌的多价卵黄抗体并检测特异性抗体的含量.采用金黄色葡萄球菌、无乳链球菌和大肠杆菌混合抗原免疫产蛋母鸡.通过水稀释法分离卵黄抗体.采用EL1SA法检测抗体效价和特异性抗体含量.多价抗体与大肠杆菌的结合效价最高可达25600,与金黄色葡萄球菌和无乳链球菌的结合效价为12800.每毫升卵黄液中大肠杆菌、金黄色葡萄球菌和无乳链球菌特异性卵黄抗体(egg yolk immunoglobulin,IgY)的最高含量分别为2.13、2.01和1.92 mg.免疫后特异性抗体含量随时间变化趋势与抗体效价变化趋势一致.

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