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论文类型：期刊论文

发表时间：2015-07-01

发表刊物：AQUACULTURE RESEARCH

收录刊物：SCIE、Scopus

卷号：46

期号：7

页面范围：1628-1637

ISSN号：1355-557X

关键字：sea cucumber; vitamin E; growth; nonspecific immunity

摘要：This feeding trial was conducted to determine the vitamin E requirement of sea cucumber (Apostichopus japonicus) and to study the effects of vitamin E on nonspecific immune parameters. Commercial sea cucumber diets supplemented with 0, 50, 100, 200, 500 or 1000mgkg(-1) vitamin E were fed to juvenile sea cucumber (7.96 +/- 0.01g) for 60days. Specific growth rate and total coelomocyte counts were maximized in sea cucumber fed diets supplemented with 100mgkg(-1) vitamin E. Reactive oxygen species production was maximized at 50mgkg(-1) vitamin E, whereas superoxide dismutase activity was the highest in sea cucumber fed diets supplemented with 100mgkg(-1) vitamin E. The vitamin E concentration in the body wall of the sea cucumber increased as the vitamin E level in the diet increased. Analysis of specific growth rate and total coelomocyte counts of the sea cucumber indicated that 88-92mgkg(-1) vitamin E is required for maximal growth and nonspecific immune responses of A. japonicus and that 114.7mgkg(-1) vitamin E is required to minimize lipid peroxidation levels.