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论文类型：期刊论文

发表时间：2012-03-01

发表刊物：AFRICAN JOURNAL OF PHARMACY AND PHARMACOLOGY

收录刊物：SCIE

卷号：6

期号：12

页面范围：864-870

ISSN号：1996-0816

关键字：Apoptosis; breast cancer; cell proliferation; cell cycle; caspase; curcumin

摘要：We examined the mechanisms by which curcumin inhibits the growth of breast cancer cells. First, we investigated its antiproliferative effects on MCF-7 cells exposed to 2.5 to 40 mu M curcumin for 24, 48 or 72 h. Curcumin inhibited cell proliferation in a dose-dependent and time-dependent manner (p<0.05) and resulted in significant cell cycle arrest in G1 phase after 72 h of treatment at concentrations of over 10 and 20 mu M in MCF-7 cells, respectively (p<0.05). In addition, curcumin caused the accumulation of cells in sub-G0 phase in a dose-dependent manner in MCF-7 cells (p<0.05). As biomarkers of apoptosis induction, caspase-3 activity and caspase-9 activity were increased by curcumin in MCF-7 cells. To investigate the effects of curcumin on the proteins regulating cell cycle arrest, cells were treated with 20 mu M curcumin for 72 h. Similar changes in the expression of regulatory proteins were detected in MCF-7 cells. Curcumin treatment resulted in decreases in cyclin D, cyclin E, CDK2, CDK4 and CDK6. The protein expression of CDK2 related to the G1 phase increased markedly with curcumin treatment. Curcumin treatment increased the expression of the CDK inhibitors p19(INK4), p21(Cip1) and p27(Kip1). Thus, curcumin exerts its anticancer effects in human breast cancer cells via cell cycle arrest at the G1 phase.